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After 2 h incubation, cells were washed twice with cold PBS and were extracted with 80% ice-cold methanol at 2 10 6 cells/mL

In traumatic injuries (neurotrauma, neuroinfection, stroke or immune attack), irreversible proliferative astrogliosis is essential for wound closure and formation of a glial border that separates fibrotic scar tissue from the healthy neural tissue and promotes postlesional regeneration

Prog Neuro-Psychopharmacol Biol Psychiatry 79:340368 Adams J, Howsmon DP, Kruger U, Geis E, Gehn E, Fimbres V, Pollard E, Mitchell J et al (2017) Significant association of urinary toxic metals and autism-related symptomsa nonlinear statistical analysis with cross validation

10.1111/j.1479-828X.2006.00543.x Aust

During and immediately after high-intensity exercise, this process is complicated by splanchnic vasoconstriction a well-documented physiological response in which blood flow is redirected from the gastrointestinal tract to active muscles and the cardiopulmonary system